Genetic factors affect the variability of fatty acid composition in ruminant products. Thus, this study aimed to investigate the association between the variation of the SCD gene and growth traits, fatty acid composition, and lipid profile in Awassi sheep. A total of 100 Awassi rams between the ages of one and two and a half years old were examined used in this study. Phenotype measurements were taken at abattoirs of Babylon, and from each animal longissimus dorsi (LD) muscle samples were taken to measure the fatty acid composition. DNA samples were isolated from each blood sample, after that PCR-single strand conformation polymorphism (PCR-SSCP) experiments were conducted for genotyping then were submitted into sequencing reactions. The study diagnosed two genotypes (TT and TA) of the SCD gene (exon 3). Several novel variants were discovered in the amplified fragments of the SCD gene. The TA genotype resulted in increased animal length and higher levels of high-density lipoprotein and monounsaturated fatty acids compared to the TT genotype. Breeding has the TA genotype and could potentially be interesting for obtaining meat containing less saturated fatty acids and more monounsaturated fatty acids, making the meat more favorable for human consumption.