Occurrence and molecular detection of huanglongbing in Bangladesh
Huanglongbing; HLB (citrus greening) is one of
The study was undertaken to get an overview of
Materials and Methods
Suspected leaves with characteristic irregular blotchy chlorosis symptom
The Las genomic DNA was extracted from midribs and petioles of symptomatic leaf using Wizard® Genomic DNA Purification Kit (Promega, Madison, USA) following manufacturer's instructions.
The samples were then examined for the detection of Candidatus Liberibacter asiaticus by DNA-based polymerase chain reaction (PCR) using the ‘Las’ specific primers “A2” and “J5” to amplify a partial sequence of β-operon (rplKAJL-rpoBC operon) of ribosomal protein genes (Hocquellet et al., 1999).
PCR products were sequenced directly in both orientations from primers A2/J5 according to the standard protocols for the ABI PRISM 3500 (version 3.7).
A band of about 703 bp specific to Las strains was obtained after PCR amplification.
Partial nucleotide sequences of ribosomal protein genes of rplKAJL-rpoBC operon showed 99% sequence identity with Las strains found in India, China, Indonesia, and USA (GenBank accession # JQ964017, DQ157278, LC090233, and KR919751, respectively).
Summary and Conclusion
The origin of Candidatus Liberibacter asiaticus in Bangladesh is uncertain.
We believe that it probably was introduced by infected material from
Results from PCR amplification and aligning ribosomal gene sequences gave strong evidence that the Las is present in Bangladesh.
It revealed the mystery of